Sentence examples for mutant constructed by from inspiring English sources
The fungus Mucor circinelloides J used in this study is a mutant constructed by carbon ion-beam irradiation to improve xylose fermentation.
Consistent with the transposon mutants (Fig. 2A), hemolytic activity was lower in a Δstp1 mutant constructed by allelic exchange and higher in the Δstk1 mutant, compared to WT Newman (Fig. 2B).
An A. caulinodans in-frame hyq operon deletion mutant, constructed by "crossover PCR", showed markedly decreased growth rates in diazotrophic cultures; normal growth was restored with added ammonium as expected of an H2-recycling mutant phenotype.
The G418 resistant rad9, rad24, chk1, pds1 and dun1 mutants (Invitrogen) were crossed with the BY4741 tlc1::HIS3 mutant (constructed by PCR-based replacement of the TLC1 sequence) to obtain heterozygous diploids.
The cbbLS::kanr mutant was not expected to grow in air because this mutant, like the cbbL insertion mutant constructed by Baker et al. [10], did not produce the carboxysomal Form I RubisCO (Figure 4) and relied instead on utilization of the available intracellular inorganic carbon pool by its Form II RubisCO.
TW30 strain is a toxR deletion mutant constructed by marker exchange-eviction mutagenesis [ 40].
One of the LxCxE binding-defective mutants constructed by our lab contains a single substitution mutation of Asn757 (RB-N, N757F), which is sufficient to disrupt the LxCxE-binding pocket [8].
Growth and development are controlled by a range of separate signalling pathways that can be probed at the molecular level by screening libraries of insertional mutants, constructed by restriction enzyme-mediated integration (REMI).
In comparison, a total of only seven mutants constructed by homologous recombination methods have been reported, two for cipA and five for other genes (ldh, pta, hpt, pyrF, and cel48S) [ 4, 18, 49, 50].
The E35Q/H300N double mutant was constructed by combining the pGTacStEH1-5H-based mutants E35Q and H300N.
The Δ139/Δ1665 double mutant was constructed by cutting and ligating appropriate parts of the Δ139 and Δ1665 mutants.
