Thus, to avoid confounding issues of structural perturbation by central segmental regions of RV RNA11, we constructed a central deletion mutant comprising the 5' and 3' termini stabilised by a central UNCG loop.
Namely, the mutations located in the 5′ UTR of PBP1, 3′ of BCS1 and in SGO1 and YNL058C seem to be linked in a single mutant that comprised a large proportion of the UV mutant population, but decreased in frequency throughout GS evolution.
We find a ratio R > 1 and CTC values beyond 2 only for two data sets – the intra-strain profiles of the fis and hns mutants, indicating that compared to wild type, in both mutants transcriptional regulation comprises a large proportion of digital-type control.
We constructed an array of 99 deletion mutants comprising all genes encoded by the virulence plasmid (excluding those known to be required for plasmid maintenance) of Shigella flexneri.
To investigate if allelic heterogeneity contributes to phenotypic variability, we analyzed an allelic series of Col4a1 and Col4a2 mutant mice comprising a splice site mutation and seven glycine missense mutations within the triple-helix-forming domains (six in COL4A1, one in COL4A2), and one missense mutation in the globular NC1 domain of COL4A1 (Fig. 1A).
We have found that the sFRP1 mutant comprising the CRD but not the NTR domain (Δ1) inhibited both AR activity and colony formation to the same extent as wild-type sFRP1, whereas the sFRP1 mutant comprising the NTR domain but not the CRD (ΔCRD) had a weaker effect in both assays.
P{A92}puc E69 mutant embryos comprise a lacZ-containing P-element inserted into the puc endogenous locus, which disrupts puc normal function.
From three F1 clutches, we sequenced the TRα TALEN target recognition site region from three individuals from each clutch with a normal and mutant phenotype (comprising a total of 79 successful sequencing runs after TOPO cloning) (Supplemental Table 2).
Using qPCR analysis, Dorshorst et al. [ 40] confirmed the duplication of both genomic regions in Fm birds, with an estimated copy number of approximately 1.5-2-fold that of wild-type individuals, suggesting that some Fm birds were likely heterozygous for a mutant allele comprising a 2-fold duplication.
However, this TF is nonessential: after it emerged in generation 2344, fitness potential of artificially generated deletion mutants comprises 98.7% of the fitness potential of wild type organisms.
