The close agreement between the Δ Tm, KMapp, ATP, and CAN508 IC50 values for the wild-type and mutant complexes indicates that the mutations do not affect ATP or inhibitor binding to CDK9 or the complex's catalytic activity.
Stability comparative studies between wild type and mutant protein-DNA complexes indicate that the former is relatively more stable than the mutant form.
All mec-10 mutant animals had properly localized channel complexes, indicating that the loss of MRCs was not attributable to a dramatic mislocalization of transduction channels.
Our kinetic data show that the GEF activity of these mutant complexes are unaffected, indicating that this interaction is not essential for the activation of the Rabex-5 GEF activity.
Absorption spectra of glucan-iodine complexes indicated distinct amylopectin structures in the mutants as compared to wild type.
Models of the WASH complex indicate that it contains a single strumpellin molecule, so in patients with strumpellin mutations, complexes containing wild-type and mutant strumpellin should be present in equal numbers.
Unlike Smc1-GFP, fluorescence associated with Smc1(M665R -GFP barrels recovers after photobleaching, indicating rapid de novo loading of mutant complexes at this location.
"Both wild type and mutant complexes displayed lifetime distributions that were fit well with two exponential components, indicating that at least two distinct diVCA-Arp2/3 complex assemblies could form".
Both wild-type and mutant complexes displayed lifetime distributions that were fit well with two exponential components, indicating that at least two distinct diVCA-Arp2/3 complex assemblies or conformations were present.
However, the identified biochemical interactions with a variety of chromatin remodeling complexes and mutant analyses indicate a much broader role for GAF [ 7- 9].
