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Furthermore, the ATPase mutant complexes contained little if any sororin, presumably due to the absence of acetylated Smc3.
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Therefore, we propose that these mutant complexes contain a mixture of b- and c-type heme species.
These results support the proposition that the mutant complex contains a mixture of heme types, a feature perhaps associated with the enhanced release function.
The first was a mutant complex containing a GFP tag in the central portion of Suz12 (Suz12-GFP269) that exhibited additional density near Lobe C. The second complex contained a C-terminal truncation of Suz12 after the VEFS domain, with a GFP tag at the truncated terminus (Suz12-ΔC-GFP).
(E ) CT7 crosslinking of mutant Sec61α/γ complexes containing the indicated cysteine substitutions.
The resulting complexes contained >90% PA7.
Consistent with the idea that complexes containing mutant strumpellin are non-functional, human wild-type, but not mutant, strumpellin could rescue the curly tail phenotype seen in zebrafish lacking strumpellin [8].
Moreover, RHOA, a known positive regulator of MAP3K1 kinase activity, exhibited increased binding to protein complexes containing mutant MAP3K1.
It is at first sight perplexing why haplo-insufficiency mutations would not also cause the disease if WASH complexes containing mutant WASH are non-functional.
The fluorescent complexes containing either mutant protein, however, failed to demonstrate localization at the cell leading edge or serum-stimulated membrane translocation.
This shows that MSL complexes containing the mutant protein are more effective at modifying chromatin immediately around sites of roX1 transcription so that the adjacent miniwhite marker has a high probability of being epigenetically activated.
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