The current Legacy is an ugly, oversized mutant compared with its Audi-rivalling predecessor.
Substantially reduced levels of H3K36me3 were observed in the set2-R255G mutant compared with wild type, indicating that this residue is required for optimal H3K36 methylation (Fig. 1d).
The fact that a smaller number of genes affected in rd26 anac019 anac055 anac102 mutant compared with RD26OX transgenic plants is consistent this hypothesis.
However, we observed a significant increase in the percentage of Sox9-positive cells in the triple mutant compared with wild-type neural progenitors.
47 genes (16 suppressed and 31 induced) showed a gain of nitrate response in the chl1-13 mutant compared with WT.
Our genetic analysis of a double mutant lacking the function of both complexes in the same pathway showed that high-B sensitivity was not additive in the double mutant compared with either single mutant (Supplementary Fig. 15).
By contrast, no difference in Pol II occupancy was observed in the crc-1 mutant (Fig. 4j), and a subtle reduction was observed in the ag-1 mutant compared with the wild type.
We found that the H2A H2B dimer became dissociated at lower temperatures (60 70 °C) in nucleosomes containing H3mm7 and the H3.3S57A mutant compared with those containing H3.3 and the H3.3T32I mutant.
We performed a quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis to confirm the microarray results using total RNA extracted from floral bud clusters up to stage 7. Similar to previous microarray data, we also detected a reduction in YUC4 transcript levels in the ag-1 mutant compared with the wild type (Fig. 2a).
In addition, the ability to synthesize GA was improved in the aba2-2 mutant compared with wild type during dark-imbibition after an FR light pulse.
Proteomic data from two-dimensional gel electrophoresis showed that 14 candidate proteins were significantly up- or down-regulated in the spl5 mutant compared with WT.
