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To infer processing functions for individual DCLs, we analyzed siR1003 accumulation in double mutant combinations of three DCLs (DCL2, DCL3 and DCL4).
Thus double and triple mutant combinations of ap2c3 and other members of the KIM-containing AP2Cs were created, but also these did not show alterations in stomata density.
We were unable to recover viable double mutant combinations of any of these three genes (tba-2 deletion alleles were not available to include in this analysis).
Double mutant combinations of rlk902 with two knockout alleles of its closest homolog RKL1 showed a further reduction of root length compared to the rlk902 single mutant.
With respect to the root phenotype, double mutant combinations of rlk902 with two knockout alleles of its closest homolog RKL1 showed a further reduction of root length compared to the rlk902 single mutant.
To avoid phenotypic expression of any unknown background mutations, we used heteroallelic mutant combinations of tam3/tam9 for pol γ−α studies and pol γ-β1/pol γ-β2 for pol γ−β.
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Single or various double-mutant combinations of single mutant alleles showed either no or only small defects in leaf growth.
To further characterize the effects of eyespot mutations on assembly of the organelle, eyespots were examined in double-mutant combinations of mini- and multiple-eyespot mutants.
The arrow indicated HA Sd coimmunoprecipitated with endogenous Brm. (C – H ) Wild-type male wings (C ) or hemizygous male wings of null allele brm 2 /+ (D ), or hypomorphic allele sd 1 /Y (E ), or double-mutant combinations of sd 1 /Y; brm 2 /+ (F ), or hypomorphic allele osa 2 /+ (G ), or combinations of sd 1 /Y; osa 2 /+ (H ).
Although best known in the context of loss-of-function mutants, combinations of other types of perturbations can also result in synthetic lethality, including overexpression of genes, the action of a chemical compound or environmental change [5 8].
Instead, they have relied on the study of single or multiple mutant combinations to reveal the generation of phenotypic patterns (e.g. [ 1]).
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