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Proline can also act as an osmoprotectant and be transported through the proP and proU systems [36], however, we could not explain the higher levels of proline in csgD mutant colonies based on these results.
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mutant colonies which were selected.
Two thermostability enhanced mutants Y187E and K205L were obtained from 1600 mutant colonies.
The selected UV mutant colonies were further screened for potential cellulase producing mutants in solid state cultivation.
Conversely, the formation and composition of mutant colonies were weak in presence of 10% ETOH compared to the low concentrations.
The PCR product was transformed to strain MS11 or FA1090 and the mutant colonies were checked using colony PCR.
Mutant colonies typically appeared 3 to 4 days later than wild-type colonies on 7H11-OADC, and 1 to 2 days later than mc2155/pVV16MSMEG_5084 colonies.
These eight mutant colonies were subjected to thermal asymmetric interlaced (TAIL) PCR [34] and sequencing.
The data is obtained by plating yeast mutant colonies in 1536-array fonmat onutrientnt media.
b Mutation frequency = (mutant colonies)/ total colonies).
Mutagenesis rate is defined as the ratio of mutant colonies to total colonies [ 5].
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