Sentence examples for mutant collection using from inspiring English sources

Double mutants were made by crossing a biofilm-deficient tpk3 :: natMX mutant with the mutant collection using a synthetic genetic array (Tong et al. 2001).

Organelle-specific GFP fusions are integrated into the mutant collection, using the synthetic genetic array technology (Li et al. 2011; Tong and Boone 2006; Tong et al. 2001; Vizeacoumar et al. 2009, 2010; Wolinski et al. 2012a).

Although the oligonucleotides for the arrays were designed based primarily on japonica sequences and the IR64 mutant collection used in this study is an indica variety, all of our comparisons are based on changes in hybridization signals relative to the wild type IR64.

We introduce an Rnr3 reporter into both of these mutant collections using R-SGA.

To conduct R-SGA, a strain containing endogenous RNR3 C-terminally tagged with GFP and expressing tdTomato constitutively from the RPL39 promoter was mated with both the non-essential gene deletion and conditional temperature-sensitive (TS) essential gene mutant collections using the SGA methodology (Tong and Boone 2006).

In fact, further scrutiny of large mutant collections collected using this strategy sometimes reveals a sizable portion (up to 20 30%) of genomes carrying a second-site mutation affecting fitness.

The phosphatase null mutant collection was screened using a chemical genomic approach with GEL, an inhibitor of Hsp90 (Gorska et al. 2012).

The sunflower mutant collection could be used for both forward and reverse genetics.

The barcodes used in this study are the well-characterized set derived from the Yeast KnockOut (YKO) collection used for screens of pooled yeast (Saccharomyces cerevisiae) deletion mutants.

The Arlequin (Alq) mutant was isolated from the screening of T-DNA mutant collections generated by using different binary vectors, the most common one included an enhancer-trapping construct [47].

E. coli BW25113 and strains from the KEIO mutant collection [ 70] were used in all experiments (Additional file 5: Table S1).