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The recent generation of an A. nidulans null mutant collection containing 103 nonessential protein kinases (NPKs) and 28 nonessential protein phosphatases (NPPs) has assisted in the dissection of the signaling pathways involved in fungal development and cell cycle (Son and Osmani 2009; De Souza et al. 2013).
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Second, US publications describing select agent research are monitored, and investigators using nonapproved markers risk mandated destruction of their mutant collections containing such markers.
This collection contains mutants in >50% of yeast essential genes and represents essential biological processes uniformly.
Thus, the reason why the original erg26 -1 mutant in the Boone lab collection contained gross chromosomal rearrangements remains unknown.
At only one locus, containing the divergent and overlapping genes MMS4 and YBR099C, did the library collection contain separate mutants containing precise deletions of each overlapping ORF.
However, the Keio collection contains two independent sets of deletion mutants and the results for some genes were inconsistent between them.
The collection contains some quirky works.
The main collection contains more than six million volumes.
The collection contains 300 plants representing about 120 varieties.
The type collection contains 1215 lots.
Collection contains two copies of item.
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