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Next, we tested whether D-cbl mutant clones display differentiation defects.
Therefore, as both the G130V and I154F mutant clones display a stable and spontaneous biochemical phenotype and are able to proliferate in classical culture conditions, these new cellular models will be useful for functional-based large-scale drug screening.
Reduced levels of ROS in cic mutant cells was not simply a secondary consequence of overcoming G1 arrest since cic mutant clones display normal patterns of proliferation in wild-type eye discs (Fig. 3A).
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A few mutant clones displayed significantly higher proliferation rate than the wild-type parasites.
Colonies producing larger depressed zones than wild-type transformants were identified as positive mutant clones displaying higher cellulase activities (see Fig. 5C showing plate of celB mutants).
Strikingly, hpo mutant clones displayed overproliferation and colonized the niche at the expense of wild-type stem cells (Fig 7C and F).
During each round of screening, only the mutant clones displaying residual activity within the first ten minutes were picked and confirmed in a second colony screen as above.
(N″ ) Mutant clones show higher levels of diap1-GFP (arrows).
Similar to mer or kibra mutant clones, which display no obvious increase in diap1 expression (Pellock et al., 2007; Yu et al., 2010), clones with α-spec RNAi showed no visible increase in Diap1 protein levels.
All the Lisp1 mutant parasite clones displayed similar growth rates in mouse blood stages and similar infectivity in mosquitoes, and yielded similar numbers of salivary gland sporozoites when compared with WT parasites (not shown).
We have shown that both dpp loss of function mutants and mad somatic clones display ectopic bristles phenotypes on the wing blade, a phenotype indicative of elevated Wg signaling.
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