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First, is it surprising that the mutant clone lingers on in a covert manner with its latent malignancy de-selected?
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It is found that a complete cycle of mutagenesis and screening, from wild-type clone to improved mutant clone requires conservatively approximately 3 6 weeks.
Thus, we decided to further characterize the ybiP mutant clone.
One MECP2 mutant clone c.916C>T (p. (Arg306Cys)) and BJ control clone were obtained from James Ellis laboratory.
The expression level of mutant clone is similar to that of WT overexpression clone.
Deletions in chromosome 11 consistent with CIN were verified in 80% of mutant clones.
γ-Irradiation at 2 Gray similarly resulted in 74.7 ± 5.7 mutant clones per 105 survivors.
The increased fluorescence intensities of mutant clones agreed with the increment of PHB amount measured by GC.
Deletion and point substitution mutant clones were generated using a QuikChange Site-Directed Mutagenesis Kit (Agilent, Clara, CA).
When compared to the wild-type phaC, about 60% of the mutant clones had lower fluorescence intensities.
Despite of that, 2% out of total mutant clones showed the distinctively improved fluorescence intensities (Fig. 5).
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CEO of Professional Science Editing for Scientists @ prosciediting.com