Consistent with this, analysis of pico mutant clones indicates that the mutant cells are extruded from the epithelium [4].
A similar rescue of Rbf1 levels is observed following removal of the presenilin gene (psn) from ept mutant clones, indicating that cleavage of a Psn substrate(s) contributes to the effect of the ept genotype on Rbf1 levels.
This notion is supported by the increased number of Rab5CA-positive vesicles in awd mutant clones, indicating a block in vesicle trafficking downstream of Rab5 function.
Furthermore, we did not observe a significant change in the expression of Sens in small or medium-sized opm 9.3 mutant clones, indicating a potential rescue effect from wild-type tissue or a partial redundancy with other p24 proteins.
Requirements for O-fucose during imaginal development were evaluated by characterizing clones of cells expressing only Ofut1 R 245 A. These clones phenocopy fringe mutant clones, indicating that the absence of O-fucose is functionally equivalent to the absence of elongated O-fucose.
(N″ ) Mutant clones show higher levels of diap1-GFP (arrows).
Therefore, a reduction in the number of INP daughter cells in bantam mutant clones could indicate increased cell death, as well as reduced proliferation.
(C, D ) Single confocal sections of haltere discs as in (A, B ), bearing pygo S123 or ssdp L7 mutant clones as indicated in panels, stained with α-Ubx antibody (red in merge); clones are marked by absence of GFP (green in merge); all discs were counterstained with DAPI (blue in merge), to label the nuclei (as internal control for the focal plane).
We did observe greatly reduced BMP signaling in tkv mutant clones as indicated by diminished pMad staining (data not shown); however, low levels of BMP pathway activity conferred by Sax-Put receptor complex appear to be enough to support ISC self-renewal (although not enough to prevent ISC from overproliferation).
Considering the role of MeCP2 as a transcriptional regulator, we wondered whether the alteration in GRID1 expression in the MECP2-mutant clone might indicate that MeCP2 protein directly binds to the GRID1 promoter to regulate its expression.
