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By contrast, following K-ras activation, the K-ras mutant clone has a higher probability of colonizing a crypt than its WT neighbors.
This mutant clone has previously been shown to be significantly more radiosensitive than the parent MGH-U1 cell line and together they provide a valuable study model.
You should make clones of Dnhe2 mutant cells in the proliferating epithelium (the eye or wing disc) and compare them to the twin-spot cloned to see whether the mutant clone has a change in cell number or cell size, and whether the labeled mutant cells have migrated away from their original location or invaded to other tissues.
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F359V could be detected at low levels at relapse post-transplant by mass spectrometry alone, suggesting that the mutant clone had not been eradicated at that time.
When compared to the wild-type phaC, about 60% of the mutant clones had lower fluorescence intensities.
In this study, most of the mutant clones had much lower or similar fluorescence intensities compared to the wild type consistent with 'fitness landscape model'.
We examined whether hth mutant clones have any effect on salm expression outside the pouch, but found no effects (data not shown).
To examine this possibility, we tested whether the cells within Asx mutant clones had assumed two possible alternative fates, wing hinge fate or haltere fate, both of which render cells that are smaller than wing cells.
Occasionally mutant clones had only sparse innervation in the antennal lobes and largely innervated non-antennal neuropile in the protocerebrum (Fig. 4D).
DNA sequencing showed that 19 out of the 21 independent mutant clones have mutations in the stf gene, with the majority of them being single-nucleotide insertion/deletion mutations occurring in regions with homonucleotide runs.
We find that FbxL7 mutant clones have only a mild effect on Ft, causing a slight decrease in Ft levels at the plasma membrane and an accumulation of Ft at the clonal boundary (Fig. 3E).
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