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We determined whether the number of myoblasts in the tw mutant changed by observing myoblasts in third instar larvae.
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As the metabolic pathways of the PGN_0274 mutant are changed by the loss of the PGN_0274 ECF sigma factor, the protein yields in the PGN_0274 mutant are more abundant than those in the wild-type and the other mutants.
Although receptor preferences of the viral mutants were changed by HA mutations in this study, almost all virus mutants exhibited attenuated infection efficiency in vitro and in vivo.
Total hydrophobicity of these mutants were changed by replacing residues of the h-domain with polyleucine or polyalanine (Fig. 2c).
However, the KM values of the mutants are changed by more than two fold for DOPA, which demonstrates that the binding affinity is changed by site-directed mutagenesis of T82.
As Figure 5B shows an exposure of the bacteria to blue light and 0.3 M NaCl for 10 min resulted in a two-fold increased infectivity of the wild type, when compared to the dark control, whereas infectivity of the Δ0799 receptor mutant was not significantly changed by blue light.
The expression level of these four transcription factors in wrn-1 gk99) wrn-1 gk99mutant not significantly changed by the addition of vitamin C (Additional file 1: Table S1).
In addition for the Phe185Cys mutant ATP potency was not changed by application of cysteine reactive MTS reagents even though they are able to bind to the receptor (Roberts et al., 2009).
Transcription levels of hundreds of genes changed by 2-fold or more in the mutants.
We explore the situation where these epidemics may be caused either by a virus and one or several mutants that changed its tropism, or by two unrelated viruses.
In addition, regarding subcellular localization of BRCA1, we carried out an immunofluorescence analysis, and found that the subcellular location of BRCA1 proteins was not changed in cells by mutant BAP1 transduction (Fig. S5).
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