He finally secured funding and selected a novel genetic approach: he would chemically induce genetic mutations in yeast cells, then isolate those mutant cells that had malfunctions in the transport and secretory system.
Moreover, RanGAP1 fails to accumulate at the nuclear pore complex in mutant cells that show a collapse in Ran distribution.
Particularly informative should be investigations of mechanisms that lead to genetic changes in hPSCs as they may reveal inherent weaknesses of mutant cells that could make them amenable to therapeutic targeting.
Therefore, loss-of-function screens with mutant cells that lack expression of a particular gene are difficult in diploid cells, because in most cases both alleles of a gene must be knocked out to result in a phenotype.
Obvious reasons for residual disease include mechanisms of secondary therapy resistance, such as the presence of mutant cells that are insensitive to the drugs, or the presence of cells that become drug resistant due to activation of survival pathways.
Therefore, we tested the docking effect of Rab3a in munc18-1 null mutant cells that are impaired in LDCV docking [21].
In this configuration, mitotic recombination produces Min/Min cells that die and ept mutant cells that populate the disc and structures derived from it.
Recent evidence suggests that stem cells may be the source of mutant cells that give rise to tumors and maintain their growth [24].
Hts (Figure 2B B″, dotted line), Integrin-βPS and Talin (data not shown) were not disturbed in ph0 mutant cells that are still integrated in the follicular epithelium.
Mutant cells that are defective in transcription elongation often show enhanced sensitivity to 6-azauracil, but fun30-deleted cells did not show this (not shown).
However, by contrast, the growth of EGFR mutant cells that harbor drug-sensitive EGFR kinase domain mutations is nearly completely blocked in this same assay, and apoptosis is induced after analogous treatment with gefitinib or erlotinib [13].
