bnl mutant cells lose their ability to constrict their apical surfaces and lose normal contacts between cells and form aberrant-shaped clusters.
The complexity of ESCRT mutant phenotypes during tissue development is maybe best demonstrated in Drosophila epithelia, where most ESCRT mutant cells lose cell polarity, which causes neoplastic transformation and non-cell-autonomous hyperproliferation of the neighboring wild-type cells.
baz mutant follicle cells lose their apical-basal polarity and form multiple layers or leave the epithelium entirely, resulting in gaps in the epithelial covering of the germline cyst (Benton and St Johnston, 2003a).
Some large (and thus PTEN mutant) cells have lost GFP expression (white arrows).
In determining the mDEL1 formation rate, only a portion of the cultures was plated so some of the mutant cells were lost.
Mutant cells also lost protection against rapamycin, a novel anticancer antibiotic first identified as antifungal (Brunton et al. 2006; Nitiss and Heitman 2007).
We found that, as with other RNAi and heterochromatin mutants, stc1Δ cells lose a minichromosome at elevated rates, are sensitive to the microtubule destabilizing compound TBZ, and display a high incidence of lagging chromosomes in late anaphase, indicative of defects in centromere function.
When shifted to non-permissive temperature during exponential growth, these mutant cells specifically lose actin cables within a few minutes [18].
In addition, wild-type cells lost mitochondrial DNA during aging, whereas shorter-lived mutant cells preserved it, excluding a causal role of mitochondrial mutations in aging.
Dmoe mutant imaginal epithelial cells lose epithelial markers and intercellular adhesion, become motile and show invasive behaviour (Speck et al., 2003).
