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We analyzed the distribution for the 18S rRNA precursors in mutant cells grown in permissive (Gal) and restrictive (Glc) conditions.
Using full-genome microarrays, we compared the expression profile of log-phase adh1 mutant cells grown on glycerol vs. glucose medium (Fig. 2b).
Wild type G. sulfurreducens and OmcZ-deficient mutant cells grown in NBAF media [22] were harvested when they reached late log phase.
Carboxysomes from wild type and mutant cells grown as 1.5 L batch cultures in air supplemented with 5% CO2 were isolated as described previously [24].
B. abortus RNA was obtained from three independent cultures of each wild type and bvrR mutant cells grown in the same conditions.
To learn more about the physiological role of LhrA, we performed a genome-wide transcriptome comparison of wild type and ΔlhrA mutant cells grown in rich growth medium until early stationary phase.
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At the non-permissive temperature, sec9-4 mutant cells grew very poorly and failed to form individual colonies, while sec9-4 vmutantouble mutant cells grew and formed colonies.
Both nrg1Δ and tup1Δ mutant cells grew as filaments under these conditions (Fig, 6 A).
However, we found that the quadruple sap mutant cells grow very poorly in sucrose media.
The G346E mutant cells grew extremely slowly on the regular SPP medium.
When compared to wild-type cells, sec9-7 mutant cells grew better at elevated temperatures than sec9-4 mutant cells.
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