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However, we found that the quadruple sap mutant cells grow very poorly in sucrose media.
A similar assay that monitors viability in the absence of adenine confirms that Fun30 is involved in silencing, because the fun30 mutant cells grow better in the absence of adenine (Figure 4C).
Thus, based on these criteria Sap4 is less functionally effective than the other three Saps and, similar to cells lacking the four Saps, cells expressing only Sap4 (sap155 sap185 sap190 triple mutant cells) grow poorly, show defects in NCR gene expression, and are hypersensitive to rapamycin and resistant to zymocin [1], [5], [15].
(C ) H4S47C mutant cells grow normally at elevated temperatures.
How can PTEN mutant cells grow at the expense of the non-mutant tissue?
In the exponential growth model, the growth coefficients and are constant, so that both resident and mutant cells grow exponentially on average.
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At the non-permissive temperature, sec9-4 mutant cells grew very poorly and failed to form individual colonies, while sec9-4 vmutantouble mutant cells grew and formed colonies.
Both nrg1Δ and tup1Δ mutant cells grew as filaments under these conditions (Fig, 6 A).
The G346E mutant cells grew extremely slowly on the regular SPP medium.
When compared to wild-type cells, sec9-7 mutant cells grew better at elevated temperatures than sec9-4 mutant cells.
We analyzed the distribution for the 18S rRNA precursors in mutant cells grown in permissive (Gal) and restrictive (Glc) conditions.
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