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Thus, vps22, vps25, and vps36 mutant cells contain abnormally large early endosomes that accumulate ubiquitin-conjugated proteins.
To examine whether anucleate compartments in cdc16-116 mutant cells contain SPBs, we visualized SPBs using the SPB marker Pcp1p-GFP, a calmodulin-binding protein, which is an essential component of SPB [19], [20].
Furthermore, the exosomes released from the KRAS mutant cells contain different proteins than non-mutant cells.
Mutant cells contain significantly less mitochondria than wild-type cells (p < 0.01).
Recent studies have found that a high proportion of Brca1 and Brca2 mutant cells contain supernumerary centrosomes [ 55**, 59**].
These mutant cells contain organelles that have a lower density, are smaller, are more abundant, and tend to cluster (Vizeacoumar et al. 2003).
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Moreover, the plasma membranes of wild-type and age3Δ mutant cells contained similar amounts of Cdr1p, Cdr2p and Mdr1p.
The Northern blot revealed that both clpX and clpP mutant cells contained rot-transcript levels that were slightly reduced compared to wild-type levels.
As shown in Fig. 4A, almost all single mutant cells contained the typical dis-like pre-anaphase nuclei (see Fig. 1A).
In contrast, heparan sulfate produced by the mutant cells contained fewer of these smaller oligosaccharides and more extended structures rich in N-acetylated disaccharides (dp>18) were present.
Some mutant cells contained large vacuoles and round-shaped lipid droplets and glycogen (Fig. 6Dc and Dd), indicating that the mutant cells might be old or in different physiological stage due to increased storage components.
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