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The reporter was derepressed in mutant cells, consistent with the effect of Dcp1.
This analysis demonstrated a 50% reduction of primary transcript in mutant cells, consistent with the ChIP data over the Scl promoter 1B.
Anecdotally it also appeared that the intensity of the LAMP1 staining of induced phagosomes might be reduced in the Cln3 Δex1-6 mutant cells, consistent with reduced LAMP1 recruitment to the phagosome membranes.
Absence of recognizable embryonic patterns in integrin α5-null mutants stained with rhodamine-conjugated phalloidin indicates aberrant distribution of F-actin bundles in mutant cells, consistent with a notion that cell polarity is disrupted in entire integrin α5-null embryos.
However, we found that BDNF completely blocked NMDA-induced activation of caspase-3 in GFP mutant cells while a significant increase (∼75%) was still observed in GFP-p75 mutant cells consistent with the lack of BDNF neuroprotection against NMDA.
In ubc7Δ mutant cells the asymmetry index of Sec61-2-GFP reanhevenn even higher value (0.27 ± 0.14, n = 71 cells) than in the single Sec61-2-GFP mutant cells, consistent with a stabilization of misfolded Sec61-2 proteins and their specific retention in the mother cell.
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However, acetylated histone H4 levels were significantly higher in the T435D cells, compared with both wild-type RelA and T435A-mutant cells, consistent with increased expression of this gene in the T435D cell line.
In HAP1 and Jurkat cells, in contrast, addition of histidinol induced ULBP1 cell surface expression slightly in WT cells, but caused decreased ULBP1 expression in ATF4-mutant cells, consistent with the aforementioned inhibition of protein synthesis resulting from histidinol treatments.
The wide variation in chromosome number in mutant cells is consistent with the large range of nuclear volumes observed (Figure 1G; Figure 5).
The finding that loss of ELG1 decreases the precocious sister separation defect in ctf7 mutant cells is consistent with our data that elg1 deletion partially recues ctf7 temperature sensitivity and in a pathway separate from Ctf18p function.
Of these, ∼23% (436, Figure 7A, group I) were misregulated both in the Mbd3 and Polycomb mutant ES cells, consistent with the observed synergy in gene regulation between the NuRD and Polycomb systems at a large proportion of target loci.
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