Sentence examples for mutant cells arrested from inspiring English sources
Furthermore, following exposure to DOX, the majority of diploid bem1Δ mutant cells arrested as budded cells and most cells exhibited cellular enlargement (swelling).
In contrast, elg1 mutant cells arrested in pre-anaphase contained a significantly higher level (20%) of precociously separated sister chromatids (Figure S2) – despite the retention of Pds1p in these cells.
However, ybp2Δ mutant cells arrested in the G2/M phase of the cell cycle (Supplemental Figure S3A), indicating that the spindle checkpoint is activated in response to unattached kinetochores.
The average spindle length in dis3-54 mutant cells arrested at 20°C (4.5 µm, Fig. 1B) was longer than that of the wild-type metaphase spindle (2.5 µm), but much shorter than the fully extended anaphase B spindle in wild-type (approximately 10 13 µm).
Because tub4 mutant cells arrested in metaphase, while wild-type cells continued cell cycle progression, we used pGal1-CDC20 cells that arrest in metaphase due to lack of activity of the anaphase promoting complex (APC) [50], as reference for metaphase cells without microtubule defects.
On the other hand, one or two cen1-GFP spots on the single pair of condensed chromosomes could be observed in the single dis3-54 mutant cells arrested at 20°C, consistent with the notion that they were arrested at a metaphase-like stage.
duo1 -S115F mutant cells arrest as large budded cells at 14°.
ndc80 -K204E mutant cells arrest with large buds and short mitotic spindles at 14° (data not shown).
We were especially interested in ESS1 interactions with cell-cycle genes because to date, there is no adequate explanation for why ess1 mutant cells arrest with a cell cycle−specific terminal phenotype (late M-phase), or what triggers nuclear fragmentation in S. cerevisiae ess1 mutants (Wu et al. 2000).
P53 mutant IGRG88 cells arrested only in G2 M and not in G0/1.
While pre-anaphase ctf7 mutant cells similarly arrested as large budded cells with Pds1p co-incident with DAPI staining, these cells exhibited a robust cohesion defect such that roughly 40% of the cells contained separated sister chromatids.
