Open image in new window Figure 3 Micrographs of WT, Δ ga5dh mutant cells, and C ga5dh cells.
The group then measured the mechanical properties of the biofilm and also looked at how the mutant cells and their scaffolding moved as the biofilm grew.
MASTR is a powerful tool that provides cell-type-specific (spatial) and temporal marking of mosaic mutant cells and is broadly applicable to developmental, cancer, and adult stem cell studies.
At the same time, the energy transfer from the PBS to the photosystem I was registered in the mutant cells and seems to occur due to the small cylindrical CpcG2-PBSs formation in addition to the conventional PBSs.
This new hypothesis is used to explain the variability in radiosensitivity observed at various cell-cycle-stages, among mutant cells and cells of different origin, or among different individuals, and to revisit unresolved issues and unanswered questions.
We included in our analyses rad61 and ctf18 single mutant cells and also rad61 ctf18 double mutant cells.
In contrast, NOT4 overexpression only modestly affected nup49-313 mutant cells and showed no effect on wild-type cells.
We also recovered ctf7 rad61 elg1 triple mutant cells and ctf7 rad61 ctf18 elg1 quadruple mutant cells.
ctf18 single mutant cells were crossed to ctf7 radoubleuble mutant cells and the resulting diploids sporulated.
We expressed Rab3a mutants locked in the GTP- or GDP-bound form in wild-type and munc18-1 null mutant cells and analyzed secretory vesicle distribution.
We hypothesized that accumulated ROS cause loss of mtDNA in atg mutant cells, and investigated ROS accumulation during nitrogen starvation using dihydroethidium (DHE).
