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The fact that expression of the UCH domain alone, but not its catalytic inactive mutant, causes an enhanced staining of AGS3 argues that the deubiquitinating activity of USP9x is both sufficient and necessary for mediating the effect of USP9x on the level of AGS3 (Fig. 4).
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In the case of horse Mb, the His(E7 Val mutant causes a 15-fold decrease in nitrite reduction rate.
The F713A mutant causes a catastrophic mechanical failure due to the loss of the hydrophobic contact between the SH2/SH1 hinge and converter.
This mutant causes a familial ALS, and could not be studied previously due to its high insolubility which is even resistant to solubilization by Triton X-100.
NapF mutant causes a growth defect under anaerobic conditions on glycerol/nitrate medium but is not essential for the activity of periplasmic nitrate reductase [ 59].
Downregulation of two PRXs in the dxr mutant causing an albino phenotype implies that those genes have roles in processing ROS produced during photosynthesis.
Downregulation of two PRXs in the dxr mutant causing an albino phenotype, implying that those genes have roles in processing ROS produced during photosynthesis.
Reducing cellulose content by the procuste1 or fei1 fei2 mutants causes an increase in lignin content [ 16, 33].
Mutation of the two potential glycosylation sites in PGU1 showed that the two residues individually (N318D, N330D) did not affect secreted enzyme activity, but the double mutant caused a 50% reduction in enzyme activity when compared to the wild-type PGU1 transformant.
The results showed Kd values of 1.4 × 10−7 mol/L and 5.3 × 10−7 mol/L for YfiBL43P and YfiBL43P/F57A, respectively, revealing that the YfiBL43P/F57A mutant caused a 3.8-fold reduction in the binding affinity compared with the YfiBL43P mutant (Fig. 6F and 6G).
The abnormal growth and loose structure of starch grain in gif1 mutant caused a significant rise in chalkiness, and the corresponding gene, GIF1 was fine-mapped on chromosome 4, which encode a cell-wall invertase required for carbon partitioning during early grain filling (Wang et al. 2008).
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