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This explanation was strongly supported by the results from transcriptional analysis (Fig. 3f) that (a): all low expressions and the existence of no discriminated difference at 20 °C regardless of WT and tcm5 mutant; (b): high expressions at 32 °C regardless of WT and tcm5 mutant despite of the higher level in the mutant caused by feedback effects.
In UAF-defective mutant caused by disruption of essential component Rrn5 or Rrn9, drastic chromatin remodeling is induced allowing access of Pol II to 35S rRNA promoter, and consequently Pol II-dependent transcription of 35S rRNA, a phenomenon called polymerase switch (PSW) occurs (Goetze et al. 2010; Vu et al. 1999).
Slow development was isolated from family 61, which is a physiological mutant caused by a conditioning lethal allele of the sw gene called swx [32], [33].
We have shown that the GM077 rice is a waxy mutant caused by down-regulation of GBSSI.
The gene is located by positional mapping using the detection of RFLPs between genomic DNAs of the wild type and dusky (dy ), a mutant caused by a transposable insertion.
We note that our current analysis cannot distinguish between differential expression in the spt mutant caused by different expression levels in the same number of cells or different numbers of cells expressing such genes at the same level.
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There were theories being propagated in Ms. Beniashvili's neighborhood: that the locusts were mutants caused by the meltdown at Chernobyl; that the snakes had been imported in train cars by some shadowy enemy; that all these natural phenomena were the result of certain explosions that happened 11 years ago on the surface of the sun.
Single-gene-knockout mutants caused by the insertion of transposable elements are of great utility to biologists.
In the present study, TGR in IDM mutants caused by intra-chromosomal recombination of a mutated target gene, was confirmed and significantly affected lethality in mice.
Cooke et al. [ 45] has hypothesized that partial protein damage has a stronger phenotype than null mutants caused by loss of gene expression.
(B ) Structure of original full-length A. boonei lysozyme expression plasmid and two spontaneous knockout mutants caused by insertion of 774 bp (mutant 1) and 768 bp (mutant 2) of IS1 transposase sequences.
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