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That mutant carries a point mutation in the aspartokinase (lysC) regulatory subunit gene as well as overexpression of d-lactate dehydrogenase (dld), pyruvate carboxylase (pyc) and malic enzyme (malE) using the strong Psod promoter.
This mutant carries a point mutation in the PLP gene, which results in severe dysmyelination and oligodendrocyte cell death [40].
The shaker2 mutant carries a mutation in the myosin XVa gene (Myo15ash2) and similar to the whirler mutant shows short stereocilia [8].
The invA null mutant carries a deletion in the translocon gene invA, rendering it unable to translocate any effector protein of TTSS-1 [41].
The slotu44c mutant carries a nonsense mutation in the hsp90α1 gene resulting in truncated molecules missing the C-terminal domain, which is important for both homo- and heterodimerization [17], [44].
The oxa1 mutant carries a double amino-acid substitution E65G-F229S in the Oxa1 protein [ 38].
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Positional cloning revealed that this mutant carries an intronic splicing mutation in the Prdm16 zinc finger transcription factor gene on distal chromosome 4.
The second mutant carries an allelic mutation in the pro41 gene encoding a protein essential for sexual development.
Every mutant carried a mutation in either marR or acrR.
The wild type Uba5 and Uba5C217A mutant carried a C-terminal HA-epitope tag where as the Uba5C217S mutant carried an N-terminal HA-epitope tag.
However, complementation could be established with a MORN1 mutant carrying a point mutation in a predicted palmitoylation site (Fig. 7B,F,G).
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