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Every mutant carried a mutation in either marR or acrR.
The wild type Uba5 and Uba5C217A mutant carried a C-terminal HA-epitope tag where as the Uba5C217S mutant carried an N-terminal HA-epitope tag.
The wild-type strain carried a H1 RFP fusion, whereas the ts mutant carried a H1 green fluorescent protein fusion.
For example, to identify virulence genes in Salmonella typhimurium, mice were infected with a S. typhimurium mutant library where each mutant carried a unique 40 bp barcode tag.
However, if the steC mutant carried a functional steC allele under the control of its own promoter on a plasmid, 94.3% ± 1.2% of microcolonies displayed an F-actin meshwork (Fig. 3A and B).
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Finally, two mutants corresponded to two distinct transposon insertions in gene FTL_1708 (the first mutant carried an insertion 216 bp and the second one, 816 bp, after the start codon of trkH).
That mutant carries a point mutation in the aspartokinase (lysC) regulatory subunit gene as well as overexpression of d-lactate dehydrogenase (dld), pyruvate carboxylase (pyc) and malic enzyme (malE) using the strong Psod promoter.
However, complementation could be established with a MORN1 mutant carrying a point mutation in a predicted palmitoylation site (Fig. 7B,F,G).
By contrast, the inhibitory effect is preserved in a HCN2 mutant carrying a CNBD deficient for cGMP binding.
This mutant carries a point mutation in the PLP gene, which results in severe dysmyelination and oligodendrocyte cell death [40].
However, a mutant carrying a deletion in a gene encoding an intestinal peptide transporter, pept-1, stores equally high levels of fat regardless of its dietary bacteria.
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