Sentence examples for mutant cannot grow from inspiring English sources

The phenotypes of these S. pombe strains indicate that the leu3Δ mutant cannot grow without leucine added to the media, whereas transformation with the plasmid containing the P. blakesleeanus leuA gene partially rescues this phenotype (Figure 5C).

Since the double ΔnrdAB/ΔnrdDG mutant cannot grow either in the presence or absence of oxygen, it was necessary to force nrdHIEF overexpression to allow its growth in aerobiosis.

Single deletion of FRD1 or OSM1 has no effect on the ability to grow under anaerobiosis, but a double deletion mutant cannot grow under such conditions unless an external electron acceptor is supplied (e.g. oxygen, menadione or phenazine methosulfate) [ 11, 12, 17].

Y. lipolytica pex23 mutants cannot grow on oleate and partially mislocalize peroxisomal proteins to the cytosol, suggesting a role in matrix protein import.

It has previously been shown that Δ ssaR mutants cannot grow intracellularly in murine macrophages or form the elongated Salmonella-induced filaments (Sifs) in epithelial cells [ 43] required for the formation of Salmonella-containing vacuoles (SCV) [ 44].

ARO2 encodes the yeast CS, and yeast ∆ARO2 mutant strain cannot grow if the three aromatic amino acids are not added to the medium (Jones et al. 1991).

This mutant strain cannot grow on ethanol/glycerol medium (YPEG: 1% yeast extract, 2% Bactopeptone, 2% ethanol, 3% glycerol, 1.5% agar) because it possesses a defective mitochondrial respiratory chain due to the inability of cytochrome c oxidase to obtain its Cu factor [ 14].

We tested this hypothesis by examining the effect of CO2 concentration on the recA fur mutant, which cannot grow in aerobic conditions because it lacks RecA a regulator of the SOS response involved in DNA strand-break repair and Fur, the main iron homeostasis regulator in E. coli (Touati et al, 1995).

This complementary experimental approach is based on employing the specific mutant yeast that cannot grow on certain media composition.

Strains of E. coli carrying a hemA mutant allele (28, 30) cannot grow on oxidisable substrates when δ-ALA is not present, a phenotype attributed to the absence of functional cytochromes, and grow only fermentatively.

The gene was cloned into an expression vector and introduced into an E. coli pabAa pabAb mutant (strain BN1163), which cannot grow on minimal medium unless it expresses a recombinant enzyme with ADC synthase activity.