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This indicates that our cyr1 Δ mutant can respond normally when cAMP is provided.
However, the fact that the AC8 N-glycosylation-defective mutant can respond to CCE, despite its localization in non-raft membranes, was quite surprising, given the current view of the stimulation of AC8 by CCE, which envisages colocalization of AC8 and CCE channels in the same lipid raft microdomain.
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While stimulation of Dync2h1 MEF cells with SAG does not increase Smo ciliary localization, stimulation of BBS mutant MEF cells with SAG further increases Smo ciliary localization, indicating that BBS mutant MEF cells can respond to Shh pathway activation, although the response is diminished.
The general conclusion from the behavior of this system is that populations can respond rapidly to selection, when mutants with small improvements are common.
Mutants deficient in the I (AHL synthase) genes do not produce the signal molecules but can respond to external signals by producing secreted factors.
We can respond".
You can respond here.
"You can respond to that.
Since the mutants originate from a directed evolution experiment and are functional, the data provide an evolutionary snapshot of how a protein structure can respond to mutations while maintaining a functional molecular architecture.
Now we can respond in three minutes".
Yes, you can respond.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com