Sentence examples for mutant by using from inspiring English sources

Construction and phenotypic analysis of a sit4 hal3 conditional mutant by using a one-step promoter substitution cassette and the Tet-regulatable dual expression system is also discussed in the chapter.

To investigate if ClpX also contributes to translation of mRNAs not interacting with RNAIII, we examined expression of the house-keeping gene rpoB in the clpX mutant by using a translational fusion between the rpoB promoter and lacZ (pLOJ133).

Transcriptional silencing could be avoided, however, in the dcl2 mutant by using progeny that were hemizygous for the GUS transgene: Run-off transcription assays showed that whereas dcl2 mutant progeny homozygous for the L1 locus were transcriptionally silenced for GUS, ones hemizygous for the L1 locus were not (Figure 1C).

Finally, we tested the catalytic efficiency of the wild-type and N44A mutant by using a cellular assay (see 'Materials and methods'), and compared these results with those obtained for an inactive mutational variant lacking the catalytic residue (E35D).

Very recently, Gonzalez et al. [ 18] reported that a large number of differentially expressed genes were identified in Spm-overproducing transgenic Arabidopsis and Spm-decreased mutant by using microarray analysis.

The actual numbers in terms of content of predicted long disorder decreased from ∼18% for the native to ∼9% for the final mutant by using the background mutation protocol (Fig. 3C, green).

The International Rice Functional Genomics Consortium IRFGCC) has generated a huge number of gene-indexed mutants by using mutagens such as T-DNA, Tos17 and Ds/dSpm.

Up to the present time, the gTME technique has been used for the construction of new mutant; however in our study we processed a new yeast strain for a library of novel mutants by using SPT15 gene.

In addition, we examined the frequency of abnormal patterning in larval muscles of tw mutants by using the MHC-tauGFP marker to observe live muscles.

Screening of ENU mutants by using fluorescent analogues revealed a mutant with impaired digestive lipid processing.

In addition, levofloxacin MICs were determined for these 2 mutants by using broth microdilution.