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Activity was partially restored in an inactivated PpENA1 mutant by the insertion of two additional serine residues on MH 4 and one on MH 6 based on the presence of these residues in fungal type IID ATPases.
Heterologous metabolic pathways were designed and established in succinic acid overproducing Mannheimia succiniciproducens LPK7 (ldhA pflD pta ackA mutant) by the introduction of heterologous genes that encode succinyl-CoA synthetase, CoA-dependent succinate semialdehyde dehydrogenase, and either 4-hydroxybutyrate dehydrogenase in LPK7 (p3S4CD) or succinate semialdehyde reductase in LPK7 (p3SYCD).
There again, complementation of the mutant by the pA3731 gene reversed this phenotype.
The double mutant was generated by the introduction of the GBAA1346 mutation into the Δ0552 mutant by the same method.
Suppression of aldicarb hypersensitivity of the eat-6 ad467 eat-6 ad467the ser-4 mutanton raises a possibylithefor a stimulatory 5-HT input to ACh neurotransmission.
Also, by week 139, when the majority of the viral clones contained the 79F mutation, the magnitude of the response was on the verge of becoming undetectable indicating that there was no cross-recognition of the 79F mutant by the GY9-specific CTL response.
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For each competition experiment we compute the relative fitness Wm of the mutant, by comparing the fold expansion of the mutant and wild type [33], [83].
Interaction between LIF2 and LHP1 was further studied in the lif2 lhp1 mutant by analyzing the expression of the four previously selected genes.
Consistently neither mutant could complement the Δ espG mutant by inhibiting the recycling of the TfR (Fig. 5E).
We scored the number of infected cells in the chamber infected with the gD mutant by determining the total area of infected monolayer.
We next examined the disulfide bond formation in wild type tyrosinase versus the Δ7 mutant by assessing the accessibility of these bonds to DTT.
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