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The ChIP experiments were performed using the extract isolated from dissected brat mutant brains that are enriched with type II neuroblasts.
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Cytoplasmic inclusion bodies containing p62 and ubiquinated proteins are present in regions of the mutant brain that undergo degeneration.
Compared with normal mice, these mutant mice had much smaller brains that contained fewer neurons because the stem cells stopped dividing earlier than normal.
Quantitative analysis of the fraction of Cux1 immunolabeled upper layer projection neurons (with respect to total NeuN+ or NeuN+Cux1-neurons) in the Nde1−/−Trp53−/− double mutant brain indicated that they were at the wild-type-level.
Analysis of cellular phenotypes in the mutant brains reveals some phenotypes that resemble natural aging in control flies, including an increase in apoptotic and necrotic cell death, axonal fragmentation, and accumulation of autophagosomes packed with crystalline-like depositions.
These mice synthesize a misfolded form of mutant PrP in their brains that shows a high tendency to aggregate but has considerably less protease resistance than conventional PrPSc, and is not infectious [17] [19].
Apart from normal vessels with a tubular structure, mutant brains displayed impaired vascular structures that appeared in EC clusters.
These results, however, indicate that dtorsin/torsin mutant brains do not have decreased levels of GTPCH mRNA and therefore have a defect in GTPCH expression at the post-transcriptional level.
An examination of labeling after a 2-h BrdU pulse administered at E11.5 reveals a similar labeling index in control (39%) and mutant brains (42%; Fig. 5 J), indicating that cell division rates are unaffected in the absence of Lhx2.
My Mutant Brain, the short film that accompanied the launch, is an almost four-minute long romp, featuring Margaret Qualley escaping the confines of a society party to do a wild dance of freedom across the art deco interiors of the Dorothy Chandler pavilion.
The abundance of p62 in 10 µg of mutant brain is comparable with that in 30 µg of wild-type brain, indicating that p62 is increased 3-fold in the mutant (Fig. 1A).
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