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Aatll was able to digest the genomic PCR products amplified from the WT, but not from the z3 mutant because of its T to C substitution in the Aatll restriction enzyme site.
We describe the KN99α rom2 phenotype as hyperelongated rather than the elongated term used to describe the S. cerevisiae rom2 mutant because of the cell elongation coupled with the lack of cell separation in this sub-population of cells.
The S1 binding pocket is narrower in the mutant because of partial occupation by the His side chain (Supplementary Figure S2 at http://www.bioscirep.org/bsr/032/bsr0320401add.htm).htm
It is therefore not clear whether c-di-GMP is elevated in the fliF mutant because of reduced levels of Pch or because Pch is inactive when delocalized.
The obligatory displacement model predicts that cortactin will be less synergistic with the GST-VCA R442A) GST-VCA R442Ae of its higher intrinsic off rate fromutantnascent becausecofplex.
The level of solvent exposure of W30 is likely increased in the W8Y mutant because of disruption of the hydrophobic core, consistent with evidence from the IR and CD spectra.
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To clarify whether the increased starch content in the ss1 L /be2b double mutant was because of enhanced substrate levels, AGPase activity in developing seeds at 10-15 DAF were quantified (Table 2).
Due to no seeds could be obtainable in homozygous mutants because of the seedling-lethality, the crosses of the heterozygous ASL3/asl3 plants with indica cultivar Pei'ai64S were conducted to generate a segregation population for gene mapping.
The ratio of docked versus moving mitochondria increases in tam3/tam9 mutants because of higher number of motile mitochondria.
However, it was difficult to evaluate the Sox1+/Nestin+ NSCs from REST-null and REST-null+MTV ES mutants, because of their low number and the ease with which they were lost from coverslips during the staining process.
They are released into the medium fraction of the lgt-mutant because of their inefficient anchoring to the membrane (Fig 2B).
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