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The genes with up/down changes in expression vs. the parental strain were grouped for each TR mutant based on their functional classification.
For each mapping population, a minimum of 30 individuals homozygous for the mutant, based on their purple phenotype, were genotyped for a series of SSR markers with known chromosomal locations.
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In addition, we selected these mutants based on their known cross sensitivity to IR as diploids [40], [39].
Paradoxically, despite the fact that vps25 clones are highly apoptotic (Figure 3B), we originally isolated vps25 mutants based on their ability to suppress apoptosis [21].
We carried out Illumina deep sequencing of the genome of several independent MMS treated ΔdinB/pdinB(D103N) or pdinB(F13V) strains that we identified as mutants based on their inability to grow in minimal medium.
A total of 13 mutants, based on their relatively low yields, were identified as sensitive candidates.
Again, the cumulative distribution plot clearly separates mutants based on their splicing efficiency.
The fspA KO cell line described in this work was isolated from a population of random insertion Dictyostelium mutants based on their inability to grow on a lawn of K. pneumoniae bacteria.
A total of 18 and 13 gene knock-outs had reproducible high-yield (enrichment p-value < 10-16) and low-yield (p < 10-6) phenotypes, respectively, and are listed in Tables 2 and 3. A total of 18 mutants, based on their relatively high biomass yield in presence of TMP, were identified as sensitive candidates.
Serine 156 or Asparagine 185 was replaced with alanine or aspartic acid, respectively, by PCR-based site-directed mutagenesis to produce the S156A or N185D AQP5 mutant based on the AQP5 pconstructonstruct as previously described [13].
We report here a Ser127Ala mutant based on the enzyme glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes.
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