Sentence examples for mutant based on from inspiring English sources
Serine 156 or Asparagine 185 was replaced with alanine or aspartic acid, respectively, by PCR-based site-directed mutagenesis to produce the S156A or N185D AQP5 mutant based on the AQP5 pconstructonstruct as previously described [13].
We report here a Ser127Ala mutant based on the enzyme glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes.
The results showed that ghd10 was a recessive mutant, based on examination of individuals of the F2 segregating population.
To design a rapid test to detect the rifampin (RIF) and isoniazid (INH) resistant mutant based on polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique that analyzes the katG, rpoB genes.
This result favors an alternative explanation to the low levels of P110 in the T192 mutant based on the genetic structure of the transposon insertion in this strain.
Donors and hosts were classified as either wild-type or mutant based on melanophore phenotype, and subsequently examined using a Zeiss Axioplan microscope with Nomarski optics, and a rhodamine filter to detect donor cells.
Mutagenesis was followed by the screening of mutants based on cerulenin as an attempt to obtain mutants displaying increased lipid productivity.
In the forward screen, we selected five highly tolerant and four highly intolerant mutants based on the response of seedlings to 300 ppm Fe.
While it would be preferable to use wild strains for wine fermentation studies, mutants based on these strains are not yet available.
The classification of mutants based on secondary structure (helix, strand, turn and coil) and solvent accessibility (buried, partially buried, partially exposed and exposed) distinguished the stabilizing/destabilizing mutants at an average accuracy of 81% and 80%, respectively for ΔΔG and ΔΔGH2O.
While numerous computational methods have been developed that use genome-scale models to propose mutants for the purpose of metabolic engineering, they generally compare mutants based on a single criteria (e.g., production rate at a mutant׳s maximum growth rate).
