We tested this by culturing eat-2 ad1116) eat-2 ad1116iquid bacteria using the BDR mutants
We also isolated several types of resistant mutants from cultures of the ancestral bacteria using selective agar plates (for methods to isolate resistant mutants, see Data S1, Mutation rates), referred to as 'first-generation' resistant mutants, and measured fitness of these mutants.
By allowing species of Salmonella to conjugate (to exchange genetic material in a kind of reproduction) in a special nutritional medium, Zinder hoped to obtain a large number of mutant bacteria to use in his experiments, conducted in the early 1950s.
To demonstrate that the proteins that bound YopH were substrates of this phosphatase we carried out dephosphorylation assays in vitro with recombinant GST-YopH produced in bacteria using as control the inactive substrate trapping mutant GST-YopH D356A.
We assayed ROS production in plants inoculated with wild-type or mutants S. meliloti, or with P. putida, or P. syringae bacteria, using 2',7'-dichlorofluorescin diacetate, which reports ROS production inside the cells [41], [42].
The bacteria used in this study were the wildtype S. Typhimurium ATCC 14028 and its isogenic sdiA mutant (ΔSTM1950: Kan-PT7) which was kindly provided by Professor McClelland at Vaccine Research Institute of San Diego, USA.
The bacteria used them to make proteins.
Bacteria used in this investigation were as follows: S. aureus Becker-MSSA (obtained from Prof Chia Lee, University of Arkansas), S. aureus Becker isdB harA deletion mutant-MSSA, and SA025-MRSA (Merck clinical isolate).
The integrity of vacuoles containing steC mutant bacteria was also confirmed using LAMP-1 labelling as a marker for the Salmonella vacuolar membrane (data not shown).
We have also clarified the rational for using mutant bacteria that is attenuated to ROS and NOS. 6) The authors claim that Perforin-2 is not required for normal murine development or for control of commensal bacteria.
