Sentence examples for mutant backgrounds were from inspiring English sources

Complementation lines on the double mutant backgrounds were developed by transformation with the vector pZP221 carrying the native promoters and the full genomic sequence for either BET11 or BET12, with a GFP tag on the N-terminus.

Expression analyses (1 and 3 dpi with S. meliloti) in symbiotic mutant backgrounds were also included in order to define groups of genes involved in early pre-infection or later infection/nodule organogenesis.

(A – C ) Relative phm (A ), dib (B ), and the ecdysone response gene e74B mRNA expression (C ) in the FoxO mutant backgrounds were quantified by qPCR.

T1 transgenic plants harboring the construct in Col-0 and irx8-5 homutantte mutant backgrounds were selected by genotyping PCR (Additional file 5) and used for complementation analyses.

Single colonies of vector-only, RcDGAT1 or RcDGAT2 transformed yeast in the H1112 and H1246 mutant backgrounds were inoculated in 10 mL of synthetic complete medium with Ura omitted and grown for 72 h at 28°C.

Profiles from mutant backgrounds are shown in red, and wild-type profiles are shown in blue.

The effect of light stress on the CRK expression in various mutant backgrounds was very different compared to the effect of the O3 response.

MC expression of flp-2 prom::gfp in dauer animals in both the wild type and ceh-19 mutant backgrounds was also compared.

Both the HU-enhanced chromatin bridge defect and a reduction in mitotic germ cells in the smc-5 (ok2421 ) mutant backgrounds are suppressed by the brc-1 (tm1145 ) mutation.

This situation, in which the removal of a very important developmental gene results in normal development in certain mutant backgrounds, is reminiscent of the case of Nanos in the early embryonic patterning of Drosophila.

For example, many Results sections finish with a raw result and a figure citation but they would benefit from one sentence of interpretation/conclusion. Also, the figures and legends need clarifying in several places such as, for Figure 4, when (and when not) FoxO null mutant backgrounds are being used in an experiment (better indicated on the figure itself).