Sentence examples for mutant background using from inspiring English sources

Finally, we overexpressed wild-type Rac1 (UAS-Rac1) in a mysnj42 mutant background using the haemocyte specific driver Hemese-GAL4 (He-GAL4) [26], and raised the larvae at 29°C to see if Rac1 hyperactivity was able to rescue loss of Mys.

Therefore, we generated transgenic lines overexpressing RACK1B or RACK1C in the rack1a mutant background using the CaMV 35S promoter.

In order to distinguish between these possibilities, we performed ceh-20 RNAi in a wrm-1 ts mutant background (using the wrm-1 ne1982ts) wrm-1 ne1982ts

Removing one copy of hid in the miR-6 miR-11 double mutant background using the Df 3L X14 recombinant produced strong suppression of the lethality.

This lethality could be rescued by restoring expression of miR-11 in the double mutant background using the miR-11 R allele.

Removing one copy each of rpr, grim and hid in the miR-6 miR-11 double mutant background using the Df 3L H99 recombinant also produced strong suppression.

We demonstrate utility by phenotyping the morphology of 12 neuronal classes in six mutant backgrounds using combinations of neuron-type-specific fluorescent reporters.

To decipher the bikaverin biosynthetic pathway and to overcome these negative regulation circuits, the structural cluster genes BIK2 and BIK3 were overexpressed independently in the ΔΔBIK2/BIK3+OE::BIK1 mutant background by using strong constitutive promoters.

Thus, we over-expressed the FgaA or FgaB transgenes in a fga1 mutant background, by using an ubiquitous heat-shock inducible gal4 driver (see materials and methods).

We therefore compared polyp formation in R482Q/+ and Fbxw7 +/− mice on an Apc mutant (Min or 1322T) background using age of developing symptoms as a measure of disease severity.

We furthermore constructed an isogenic in-frame flaR null mutant in L. monocytogenes 10403S background using Splicing-by-Overlap (SOEing) PCR and allelic exchange, as previously described [ 69] for use in swarming assays.