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On the other hand, in the suxA deletion mutant background, the induction was linear and less rapid.
We then studied in the same mutant background the subcellular distribution of Nrg-GFP [18] and Dlg [19], two SJ markers.
In the double mutant background, the presence of the fadD1 mutation suppresses the phenotype of the fadD2 mutation (Figs. 6 and 7).
However, in the ft-10 brm-2 double mutant SOC1 is slightly up-regulated, indicating that SOC1 is also involved in this phenotype and that BRM is able to repress SOC1 independently of FT. Besides, in a co mutant background the brm early-flowering phenotype is partially rescued.
The best argument that the Min system is involved in divisome disassembly stems from the fact that in a minJ mutant background the septa at the used sites of septation are spiral-like (or elongated, as it was described in E. coli [41].
In the mutant background, the DVC cell body, as identified from the GFP expression, remained in the correct position in the tail ganglia, as examined by DIC microscopy.
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In control and mutant backgrounds, the number of GFP-labeled mitochondria that crossed a specific point across the nerve were counted, and the total was then divided by the 'time of observation' to yield 'flux' in terms of mitochondria/min.
To express Ofut1 R 245 A in Ofut1 mutant backgrounds, the MARCM technique [ 27, 28] was used.
Third, in several different mutant backgrounds, the rate of alterations in the number of repeats is very high, greater than 10−3/division (McCulley and Petes 2010; Song et al. 2014).
While it is tempting to speculate that unwinding of G-quadruplexes preserves genomic stability, it may be that in defined genetic mutant backgrounds, the action of G4 DNA helicases have detrimental effects on genome homeostasis.
However, transgene expression in the mutant background rescues the phenotype [29].
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