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However, transgene expression in the mutant background rescues the phenotype [29].
Importantly, we found that soma-specific reduction of DE-Cad or Arm levels in ecdysone mutant background rescues early germline differentiation phenotypes (Fig. 5A), while overexpression of DE-Cad in ECs phenocopies ecdysone signaling phenotype (supplementary material Fig. S4E); together demonstrating that ecdysone signaling and DE-Cad/Arm interact in the ECs.
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Expression of a wild-type ETR1 transgene in the etr1-9 ers1-3 etr1-9 ers1-3 etr1-9 ers1-3ll the phenotypes we observed in the etr1-9;ers1-3 double mutant.
First, on a TK mutant background, rescuing TK expression only within the NPF circuit did not restore the courtship suppression response.
Expression of wild-type SCAR specifically in hemocytes in a SCAR Δ37 mutant background (SCAR rescue) significantly rescued hemocyte speed, demonstrating a cell autonomous requirement of SCAR for effective migration.
(B ) KC expression of DopR1 in the dumb 2 mutant background fully rescues the LTM impairment (n = 12, 10, 7, 13).
We now include the results from this experiment in Figure 4. We find that expressing ppk29 in R58F10 in a ppk29 mutant background partially rescues the larval attraction to (Z -7-tetradecenoic acid, but not (Z -7-tetradecenoic acid.
In addition, hemocyte-specific expression of wild-type SCAR completely rescued developmental migration defects in a SCAR Δ37 mutant background (SCAR rescue), further demonstrating an autonomous requirement for SCAR.
Furthermore, reduced expression of PHE1 in a mea mutant background can rescue the mea seed abortion phenotype [42, 43].
ena overexpression in the mutant background specifically rescued the behavioral phenotype.
The individual transgenes (HS-GAL4, elav-GAL4, RS-GAL4, UAS-OAMB, and UAS-CaMKIICA) were placed in the oamb286 or oamb96 null mutant background for rescue experiments.
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