Sentence examples for mutant background of from inspiring English sources
Interestingly, the genic CHG methylation is also induced in the mutant background of CG methylase gene MET1 (Cokus et al, 2008; Lister et al, 2008).
The chromosomally integrated transgenic array leIs2703[ceh-19b prom:: gfp, unc-119] was crossed from strain UL2703 into the ceh-19 tm452) ceh-19 tm452round of UL3128 to give strain UL3413.
In addition, the extrachromosomal array leEx3025, carrying the recombineered fosmid with the ceh-63 protein coding region replaced with gfp, in the strain UL3025 was also crossed into the ceh-63 tm541) ceh-63 tm541round of UL3122, giving strain UL3161.
The extrachromosomal array leEx3011 and leEx3012 both carrying the recombineered fosmid fUL HF005.1 with the ceh-19b protein coding region replaced with gfp, in the strains UL3011 and UL3012 respectively, were also crossed into the ceh-19 tm452) ceh-19 tm452round of UL3128 to give strains UL3013, and UL3019 and UL3022.
To address the evolutionary conservation of COP1 and SPA protein sequences, we expressed COP1 and SPA coding sequences from rice and Physcomitrella in the respective cop1 and spa mutant backgrounds of Arabidopsis.
This is shown in Figure 4 and in the non-mutant background of Figure 7 (compare panels A and C).
The result also suggested that either of these genes might be exploited as a basis for a sensitized background to screen, ideally in a viable mutant background, for enhancement of embryonic lethality, a readily scorable phenotype.
Both strains are in a white-eyed pearl mutant background to facilitate detection of eye-specific fluorescence.
Thus, N. benthamiana provides a natural mutant background for exploring the effect of AOX on plant responses to virus infection without the potentially confounding presence of active RDR1.
We then examined embryo cross-sections for spreading defects in loss-of-function mutant backgrounds for this set of genes.
Importantly, deletion of ELG1 significantly reduced the incidence of separated sister chromatids in the ctf7 mutant background – regardless of the pre-synchronization strategy employed (Figure 3).
