Sentence examples for mutant at position from inspiring English sources
For site-directed mutagenesis, the following primers were utilized: Sebox 5 (new_mutant at position 2 generating the second G): 5′ GATGATGAACTC TTCACgTCCCACACG 3′; Sebox 6 (new mutant at position 3 generating the first E): 5′ GATGATGAACTCTTtACCTCCCACACG 3′; Sebox 7 (new_mutant at position 4 generating the second E): 5′ GATGATGAACTtTTCACCTCCCACACG 3′.
One hundred alternative conformations of each hemoglobin mutant at position 122β position were generated by the program.
In 4 isolates, a mixed population of wild type and mutant at position 2063 were identified on sequence chromatograms.
Peak positions from the anomalous electron density maps for the three derivatives (see Figure 4B ) are shown and colored as follows purple (4-iodophenylalanine mutant at position 14), orange (diselenide mutant of Cys7-Cys28), and yellow (SeMet mutant at position 29).
Control simulations included one system in which the STX is immersed in a PIP2-free membrane model (i.e., POPC), and another in which a lysine-to-alanine mutant at position 2, STXK2A, is immersed in the PIP2-containing PM membranes.
Thus, in order to evaluate our hypothesis on the significance of α-proton accessibility on the fate of sulfonium, we expressed and treated a single cysteine mutant at position 230, GFP(T230C, 233Δ) 6, with 2,5-dibromohexanediamide (4, 50 eq., 2 h, 37 °C).
The library included the following 73 peptides that represent the wild-type sequence and each possible mutant at positions 7 10 of histone H3: TAX8KAcSTGGK14APC, TARX9STGGK14APCPC, Tand9STARKAcX10TGGK14APCKAcX10TGGK14APC, where X is substituted with any of the natural amino acids except for cysteine.
Replacement of Tyr224 by phenylalanine results in inactive enzyme, whereas mutants at position Arg373 retain catalytic activity close to wild-type level.
Further, pH-activity profiles of mutants at position 91 and 226 were analyzed.
In addition, the mutants at position 114 have been shown not to regenerate with 11-cis-retinal [33], [39].
Four mutants at position 139 were tested.
