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All these data indicate that innate immune responses are sufficient to kill the mutant, as seen in the resting macrophages which produced limited oxidative burst [37].
This 19% reduction in the Kcat correlates well with the minor loss of structure for this mutant as seen in Figure 3, which is also 18%.
Further, viral replication compartment formation was also hampered by the dominant negative Hsc70 mutant, as seen in the cells stained with ICP4 and ICP8 antibodies, and which also expressed GFP-Hsc70 K71M (Figure 9B).
Double mutants in gacA and lasR behave similar to the lasR mutant, as seen by abrogation of a characteristic cell type specific host cell damage caused by PA14 or the gacA mutant.
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(B ) Morphology of mechanosensory neurons in wild-type and ptrn-1 mutants, as seen by expression of mRFP under the control of the mec-7 promoter.
In the L1 stage, we observed low levels of perinuclear P granules in the PGCs of meg-1 meg-3 meg-4 triple mutants as seen in meg-3 meg-4 double mutants.
Instead of being a drawback, the presence of multiple melting domains generates a particular curve morphology that differentiates mutant and wild type melting patterns more clearly, even with low amounts of mutant DNA, as seen in the KRAS assay.
In the case of 6% (for forward primer) and 3% (both forward and reverse primer) mutant DNA in wt DNA, sequencing analysis was not able to incontestably confirm the presence of mutant alleles, as seen by the nucleotide sequence generated by the sequencing software.
A mode of inheritance that is dominant with incomplete penetrance allows for a high frequency mutant alleles as seen in this pedigree.
The lack of beta-oxidation leads to higher amount of stored fat in nhr-49 mutant worms, as seen in nhr-49 ok2165 nhr-49 ok2165d S10Figs
The L80F mutation dominates over L58F mutation in case of the double mutant (L58,80F), as seen by its overall effect on SAP activity.
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Justyna Jupowicz-Kozak
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