The number of bulliform cells is obviously increased, but the size of middle bulliform cells is significantly decreased in leaf blades of rel2 mutant as compared to WT.
Among the 134 intracellular metabolites that we identified, the 98% reduction of sedoheptulose was found to be the most significant change in the pho13Δ mutant as compared to its parental strain.
Unexpectedly, these compounds showed a strongly reduced dissociation rate from the K103N mutant, as compared to the wild type enzyme, suggesting that, once occupied by the drug, the mutated binding site could achieve a more stable interaction with these molecules.
Biosynthetic genes are dramatically more reduced in expression in the myb29myb28 double mutant, as compared to the single mutants.
Root growth was also slower in the mutant as compared to the WT (Figure 4E and F).
We calculated the average fraction of calpain cleavage observed for each mutant as compared to WT cleavage.
This could explain the shorter lag phase observed when AChE586-599 was co-fibrilized with the F3/A mutant, as compared to AChE586-599 alone.
However, 78 genes were expressed even higher in the eed1Δ + pTET-UME6 mutant as compared to the wild type (Fig. 6 B, Table S4).
The expression profile of transporter and adhesion proteins revealed significant changes in the ecsA::intron mutant as compared to the wild-type strain.
Results (Fig. 3) showed a significant (P≤0.05, n = 10) decrease of the number of adherent cells with the mutant as compared with the wild-type.
These genes were identified as such when meeting the following three criteria: 1) induced by growth in high iron as compared to low iron media, 2) de-repressed in the ΔprrF1-2 mutast as compared to wild type when grown in low iron media, and 3) unchanged in the complemented ΔprrF1-2 mutast as compared to wild type in low iron media.
