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Other genes significantly de-repressed in this mutant are those under the effect of regulators of stress elements (Msn2/4 and Snf1), sporulation (Snf1, Ras2), nitrogen starvation (Ras2) and chromatin remodeling (Snf2, Swi1).
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The lesions that are not repaired in recombination mutants are those that affect both DNA strands in the same location.
The third group of mutants are those that make important intra-chain contacts and are probably required for the stability of the TIR domain fold.
Among the pathways affected by o2 and o7 mutants are those leading to the synthesis of the aromatic (Phe, Trp, and Tyr), Asp-derived, and BCAA aminoacids.
Among these hypersensitive mutants are those that fail to show the characteristic response to Microbacterium nematophilum infection, a swollen rectal epidermis; such mutants have the " Bus" phenotype (bacterially un swollen).
Unsurprisingly, the most up-regulated genes under iron-replete conditions in the isogenic deletion mutants are those previously characterized as being involved in iron acquisition (Fig. 2 [Cluster G]) or in oxidative stress defense (Fig. 2 [Cluster H]).
Electroporation of S. suis and procedures for isolation of mutants were those described previously [50].
These mutants were those altered in the eleven residues preceding the first TM (e.g. Gap1-126) or in intracellular loops L2, L4 and L6 (Table 1 and Fig. 3).
However, morphology and other features of this mutant are not those of typical fully differentiated bradyzoites (Figure 6B-D).
The overall distributions of the reduced spectral densities J ωN), J 0) and J 0.87ωH) for the I214V mutant are similar to those for the wild-type reported previously [30].
In particular, the levels of total and photoactive Pchlides in fgl mutant are significantly lower than those in WT, which is consistent with the reduced size of PLBs in fgl mutants (Sakuraba et al. 2013).
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