Following co-electroporation, mixed plaques were identified, and a homogenously pure mutant was readily recovered (Table 3).
We propose that CBS1 is required for normal peroxisomal biogenesis, but that this mutant is readily suppressed, the mechanism of which remains unclear.
As expected, skin biopsy cultures indicated that the WT and the complemented mutant were readily transmitted to the dermis of naive mice by infected nymphs (Table 2).
While the isogenic BbCRASP-1 mutant were readily killed within 1 hour of serum exposure, the viability of the BbCRASP-2 mutant did not differ significantly from that of the wild type (Fig. 4) indicating that BbCRASP-2 is not essential for B. burgdorferi resistance to complement-mediated lysis in serum.
Later during seedling development, the difference between the wild type, lrx3 lrx4 double, and lrx3 lrx4 lrx5 triple mutant was readily detectable in terms of size and shape, excluding that the mutants just grow slower than the wild type (Additional file 2A).
Our data show that, similar to the wild-type HSF1 protein, the L189P mutant is readily hyperphosphorylated in response to heat stress-exposure and suggest that a different aspect of the HSF1 activation pathway is affected by the L189P mutation.
A deletion mutant was readily obtained in a screen of 50 colonies, and only two rounds of purification on low osmolarity defined (LOD) plating media containing uracil [ 27] were required to resolve the merodiploid, resulting in C. hydrothermalis JWCH008 (ΔpyrF ΔchyI).
We chose ERG3 for further study, as specific phenotypes previously characterized in yeast erg3 mutants are readily complemented.
All MT2 mutants were readily detectable at the cell surface (Figure 2B).
Growth of S. cerevisiae on oleate requires intact peroxisomes; hence peroxisome-deficient mutants were readily selected from collections of oleate utilization-deficient mutants (Erdmann et al. 1989).
