We also confirmed that adding terminal CCA to Methanococcus jannaschii tRNATyr mutant is important to its toxicity against Escherichia coli.
These altered function mutants are important for identifying essential genes in which larger mutations would be lethal.
Thus, we detected the 36 heteroclite mutants (P <0.05, Bonferroni correction; Additional file 6: Table S2) with the first four PCs, expecting that the gene functions disrupted in these 36 mutants are important in nature.
We do agree with the reviewer that additional literature demonstrating amyloid clearance upon repression of synthesis of the amyloidogenic protein, as well as the effects of dominant-negative mutants are important to consider as well.
Therefore, evaluation of the differences in dynamics between wild-type PrPC and its mutants is important to address the molecular mechanism of prion conversions.
Determination of the mutant prevention concentration (MPC), the antimicrobial concentration that prevents selection of resistant mutants, is important for reduction of the incidence of fluoroquinolone resistance [ 4].
Isolating such mutants is important because continuous TAG degradation could occur and limit oil build up in Chlamydomonas as was shown in plant leaves [ 37].
The comparison of PEX5 and PEX13 mutants is important in terms of the projected roles of the brain and extra-neuronal tissues in cerebellum development.
The conclusion that gain-of-function in p53 mutants is important for tumorigenesis is strongly supported by the results of bioinformatic analysis of the mutation spectra of the p53 gene [ 25, 26].
We reasoned that functions of the IDH mutants that are important in cancer will be shared between all eight mutants, and that functions that are not required for cancer pathogenesis may not be shared.
