A distinct phenotype of the Δflv mutants appeared only under LC (Figure 5C and 5D).
We estimated, given this capability, that we could sequence 25 250 bp fragments in two directions from each of the ~18,000 haploid genomes in the library at sufficient coverage to detect multiple reads of a mutant allele that appears only once in a single 288-fish library pool.
The A1552∆hapR derivative was strongly attenuated when compared to the wild-type strain A1552 whereas the A1552∆prtV mutant strain appeared only moderately attenuated (Fig. 1a).
However, only the WT females showed clear signs of milk-filling, displaying large, distended alvoeli after 2 hours without pups, panel c, arrows), while glands of mutant mothers appeared only slightly distended, panel d).
It appears only with the teacher.
Remember, Jackson appears only during daylight hours!
Under schema 𝒜, we see similarities between the structural distribution of WT, m4, m3, m9, and m11; however, the toxic m8 mutant appears to strongly prefer only one of the 2-rung conformations.
Also, only the DRY/AAY mutant appears severely impaired in signaling in our setup.
In inx-21 ok2524) inx-21 ok25244316) mutands, inx-21 tm4316zation inx-21 tm4316 mutantslly affected; however, we show below that these are not null alleles of INX-22.
Each mutant appears in the population at frequency 1/ N. Realistically, population sizes fluctuate and only a subset of cells actively divide.
