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Only a small fraction of mutant animals, however, exhibited hemorrhages (Figure 6 E and F).
We also saw absence of collateral presynaptic varicosities in mec-17 mutant animals, however this phenotype was additive in mec-17, dlk-1 double mutants indicating that mec-17 and dlk-1 have parallel presynaptic roles (data not shown).
Prohibitin deficiency shortens the lifespan of p53 (cep-1) mutant animals, however, it would be necessary to examine the requirement of p53 in the cases where PHB deficiency extends lifespan.
The disparate behavior in the circadian mutant animals, however, implies that the contribution of distinct clock proteins to this cognitive process extends beyond that of local timekeeping in the brain.
In Ret mutant animals however, C4da neuron dendrites already showed defects at 48 hr AEL (not shown) and 72 hr AEL, as evident from incomplete coverage and abnormal dendrite patterning at that stage.
Examination of representative slices from mutant animals, however, shows strikingly different characteristics; whereas the WT SCN shows distinct and stable patterns of expression that progress through the circadian cycle (Fig. 1, top row), the Cry‐null SCN does not oscillate, instead it shows continuous expression in a stable spatial organisation throughout the circadian cycle.
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Our results reveal that the structure of the SC in mutant animals is intact; however, it is significantly thinner with an apparent fusion of the intermediate grey and white layers.
As shown in the present study, however, mutant animals deficient in odr-3 or gpa-3 showed similar avoidance indices to those of wild-type animals.
Colonic activity consisted of periodic large amplitude phasic contractions occurring at ∼3 cpm in WT controls (Figure 4C and G) and mutant animals (Figure 4D and H); however, phasic activity was less organized in mutant mice compared to the controls.
However, buffy mutant animals did not have more mitochondria since no increase in mitochondrial genomes was observed in larval or fat body extracts (unpublished observations, JPM and CBB).
However, sterile mutant animals that are defective in sperm production (Klass 1977; Kenyon et al. 1993; Arantes-Oliveira et al. 2002), or in which oocyte precursor cells undergo apoptosis (Arantes-Oliveira et al. 2002) display similar life spans to fertile WT animals.
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