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Indeed, fbf-1 fbf-2 double mutant animals fail to make oocytes, which results in germline masculinization (Crittenden et al. 2002).
Furthermore, hif-1 (ia04 ) mutant animals fail to increase sqrd-1 and cysl-2 mRNA levels during RA/H2S exposure, suggesting that upregulation of sqrd-1 by HIF-1 is a mechanism for surviving in the presence of H2S.
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Specifically, mutant animals failed to develop BAT levels appropriate to body weight at P14 (Table S1).
We found that mutant animals failed to construct proper nests (Z = −3.4; P = 0.001) (data not shown).
Unfortunately, these mutant animals typically fail to survive for > 24-48 hr after birth.
By contrast, in almost all of the daf-18 mg198) daf-18 mg198ls AIY failed to reach the dorsal mutante (144 out of 145 animals exAIYned had AIY neurites that did not reach the dorsal midline) (failedC,D).
Most animals fail the first step, reacting by instinct.
Another class of mutant animals that might fail to be identified by this screen are mutants that cannot sense food or a chemical odorant.
We used five GFP-based cell fate markers to characterize the vulva phenotype in mutant animals and found that the cells in hda-1 animals failed to acquire correct identities.
Although we kept the runt mutants with their mothers beyond the normal weaning age, the animals failed to thrive and remained extremely small.
Examination of more than hundred crypts per animals failed to revealed doubly stained cell (not shown), strongly arguing against mutant cells elimination by an apoptotic process during mitosis.
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