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First, mutant animals exhibit sensitivity to gamma irradiation.
The mutant animals exhibit malfunctions in systemic calcium homeostasis and develop rickets [11].
GA25 mutant animals exhibit the largest number of white cells, suggesting that it is the weakest of the missense mutations (Figure 4D).
Mutant animals exhibit lower efficiency of double strand break (DSB) repair as assayed by reconstitution of the DsRed transgene in vivo [46].
These lin-12(n137) mutant animals exhibit a Multivulva (Muv) phenotype (the mutants possess 2 6 vulval protrusions, whereas wild-type hermaphrodites have normally one vulval structure, which does not protrudes from the ventral surface of the body).
This could explain why the mutant animals exhibit deficits in one trial learning which involves rapid plastic changes in hippocampal cellular assemblies (Nakazawa et al., 2003).
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The mutant animals exhibited bipolar disorder-like phenotypes, such as a distorted day night rhythm and a robust activity change with a period of 4 5 days, and the behavioral abnormalities were improved by lithium.
Dlk1 was abundantly expressed in the pituitary at this stage, with mutant animals exhibiting Dlk1 overexpression.
In contrast, mutant animals exhibited lethality at various stages depending upon the gene and allele.
Finally, by comparing the number of immobile episodes in DD conditions, no significant differences between genotypes were detected during the subjective day whereas during the subjective night mutant animals exhibited a significantly lower number of immobile episodes (F1,108 = 44.68; P < 0.001, Fig. 3f).
Taken together, our results show that the postnatal cortex of double-mutant animals exhibits a great and specific reduction in the number of cortical MGE-derived interneurons.
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